Applied Biosystems

Company Overview

Applied Biosystems is focused on basic research, commercial research (pharmaceutical and biotechnology) and standardized testing, including forensic human identification, paternity testing and food testing. The company has an installed base of approximately 180,000 instrument systems in nearly 100 countries.

Basic research includes work at university, government and other non-profit institutions that focus on uncovering the basic laws of nature and understanding human disease. Pharmaceutical and biotechnology companies use Applied Biosystems' products to discover and develop new drugs more effectively. Standardized testing customers require systems that produce precise results from a high volume of automated tests.

Quick Facts
Applied Biosystems
850 Lincoln Centre Drive
Foster City, CA 94404

Web:	www.appliedbiosystems.com

Phone:	1 (650) 638-5800
Fax:	1 (650) 638-5884

Featured Solutions

• TaqMan gene expression assays for validating hits from fluorescent microarrays by Applied Biosystems
  This white paper by Applied Biosystems describes TaqMan gene expression assays, which are designed differently than microarrays and have no positional bias within a transcript.
  
  

• Analysis of Complex Low Level Samples Using Cleavable ICAT Reagents, ... by Applied Biosystems
  In this application note, Applied Biosystems describes how the use of a new cleavable ICAT reagent combined with multidimensional liquid chromatography (MDLC) and two modes of mass spectrometry have enabled the identification and differential profiling of low level proteins from complex samples.
  
  
• Multiplex protein quantitation in saccharomyces cerevisiae by Applied Biosystems
  In this application note, Applied Biosystems explains the use of the new isobaric multiplexing tagging reagents, iTRAQ reagents, in the global protein expression analysis of a series of deletion mutants of <I>saccharomyces cerevisiae</I>.
  
  
• Protein quantification using stable isotope tags and a hybrid ... by Applied Biosystems
  In this application note, Applied Biosystems provides the technical data on a method for quantitation of specific cytochrome P450 proteins in liver using ICAT reagents and a hybrid quadrupole linear ion-trap mass spectrometer.
  
  
• Essentials of real-time PCR by Applied Biosystems
  In this application note, Applied Biosystems describes real-time polymerase chain reaction (PCR) and two types of chemistries the company has developed for detecting PCR products.
  
  
• Multiplexed quantitative peptide assays for protein biomarkers of ... by Applied Biosystems
  In this application note, Applied Biosystems describes developing an assay using multiple reaction monitoring (MRM) and MIDAS workflow on the MIDAS TRAQ system to detect and quantitate selected tryptic peptides, where each peptide represents a specific candidate protein biomarker identified for cardiovascular disease.
  
  
• Reconstituting and diluting primers and TaqMan probes by Applied Biosystems
  In this application note, Applied Biosystems describes the reconstitution of TaqMan lyophilized primers and probes to a working stock concentration which later can be distributed into single-use aliquots to extend their life.
  
  
• Protein quantification of plasma and serum using iTRAQ reagent labeling by Applied Biosystems
  In this application note, Applied Biosystems explains a strategy to aid in the identification of potential biomarkers from serum or plasma by the use of iTRAQ reagents combined with 2D LC MSMS.
  
  
• Total RNA purification from whole blood by Applied Biosystems
  In this application note, Applied Biosystems describes its newly developed techniques that produce the highest-quality total RNA from whole blood or isolated blood cells from various animal species and a variety of anticoagulants, including heparin which allows to isolate highly pure, highly stable total RNA directly from whole blood, without sample pre-treatments such as proteinase K digestion or red blood.
  
  
• Biomarker discovery on the 4800 MALDI TOF-TOF analyzer by Applied Biosystems
  In this application note, Applied Biosystems describes its MDS SCIEX 4800 MALDI TOF-TOF analyzer and iTRAQ reagents for the discovery of putative biomarkers for anaplastic oligodendrogliomas in human brain tissue.
  
  
• Real-Time PCR advantages over traditional PCR by Applied Biosystems
  In this application note, Applied Biosystems discusses the evolution of traditional PCR methods towards the use of Real-Time chemistry and instrumentation for accurate quantitation; and the advantages of real-time PCR over the traditional PCR.
  
  
• Simultaneous determination of psychotropics in blood using Q TRAP system by Applied Biosystems
  In this application note, Applied Biosystems discusses its newly developed method using liquid chromatography tandem mass spectrometry for simultaneous determination of psychotropic drugs in human blood using the quadrupole linear ion trap mass spectrometer Q TRAP system.
  
  
• Efficient primary structure elucidation of disulfide-bridged peptides by Applied Biosystems
  In this application note, Applied Biosystems explains how the primary structure determination of disulfide bridged peptides is greatly facilitated using a combination of the Procise cLC 492 sequencing system and the QSTAR pulsar hybrid quadrupole time of flight mass spectrometer.
  
  
• Simultaneous screen of 23 drugs of abuse by LC-API MS by Applied Biosystems
  In this application note, Applied Biosystems discusses a LC-MS/MS method with fast scanning which can simultaneously screen 23 drugs of abuse, and present the results in simple format for interpretation by interested parties.
  
  
• ProteinPilot software for protein identification and expression analysis by Applied Biosystems
  In this application note, Applied Biosystems explains its ProteinPilot software for performing protein identification and relative protein expression analysis for discovery research.
  
  
• SQL LIMS software support for the FDA electronic records rule by Applied Biosystems
  In this application note, Applied Biosystems outlines the key areas of the FDA issued electronic records rule and how they are supported by SQL*LIMS software which provides support for both audit trail and change control for procedures.
  
  
• Rapid liquid chromatography and fast MS-MS acquisition enables high ... by Applied Biosystems
  In this application note, Applied Biosystems explains the QSTAR elite system coupled with the Tempo nano LC system which provides rapid, robust protein identification.
  
  
• Strategies to identify and confirm metabolites using the 4000 Q TRAP System by Applied Biosystems
  In this application note, Applied Biosystems describes the superior sensitivity of 4000 Q TRAP System that provides ultra low-level detection of metabolites, which combined with the variety of scan modes facilitates metabolite identification capabilities which is best suited for Phase I and II metabolite screening where the lists of expected metabolites are easily compiled.
  
  
• Tools for use with the ABI 3900 DNA Synthesizer by Applied Biosystems
  In this application note, Applied Biosystems describes the tools that can be used on the ABI 3900 DNA Synthesizer for the analysis of proteins.
  
  
• Total RNA purification from cultured cells using the ABI PRISM 6700 by Applied Biosystems
  In this application note, Applied Biosystems describes its newly developed techniques using the ABI PRISM 6700 Automated Nucleic Acid Workstation that produce high purity total RNA from biological sources such as cultured cells which eliminates the need for post-isolation DNase treatment or other manual handling, and produce a highly pure product, free of PCR inhibitors, that is less prone to degradation.
  
  
• Validation of identifications of low abundance proteins using the MIDAS workflow by Applied Biosystems
  In this application note, Applied Biosystems presents an approach for the validation of low abundance protein identifications using targeted LC/MS/MS experiments on the 4000 Q TRAP LC/MS/MS system.
  
  

• 4000QTRAP LC/MS/MS System by Applied Biosystems
  Delivering maximum sensitivity quantitative and qualitative performance in one instrument, the new 4000 Q TRAP LC/MS/MS System opens up a whole new class of application workflows for proteomics, drug discovery, and drug development.