GE Healthcare Bio-Sciences

Company Overview
GE Healthcare Bio-Sciences is a $3 billion business of GE Healthcare that provides diagnostic pharmaceuticals, drug discovery tools and protein separation systems. Protein separation systems purify biopharmaceuticals used in the research, development and manufacturing stages of more than 90 percent of the biopharmaceuticals currently on the market. GE's drug discovery tools enable functional biology to help bring more effective drugs to patients through enhanced understanding of cellular and protein analysis, DNA sequencing, gene expression and genetic variation.

Quick Facts
GE Healthcare Bio-Sciences
800 Centennial Ave.
P.O. Box 1327
Piscataway, NJ
08855-1327

Web:	www.amersham.com

Phone:	(732) 457-8000
Fax:	(732) 457-0557

Featured Solutions

• Optimizing Elution Conditions on Capto MMC Using Design of Experiments by GE Healthcare Bio-Sciences
  In this application note, GE Healthcare Bio-Sciences describes how design of experiments (DoE) can be used to optimize elution conditions on Capto MMC ion exchangers and thus recovery of a target protein.
  
  
• Oligonucleotide purification by ion exchange chromatography by GE Healthcare Bio-Sciences
  In this application note, Amersham Biosciences, now GE Healthcare Bio-Sciences, describes how purification method development, optimization and scale-up of a 20-mer oligonucleotide is done by anion exchange chromatography on SOURCE 30Q.
  
  
• Factors affecting the accuracy and reproducibility of gradient formation by GE Healthcare Bio-Sciences
  In this application note, GE Healthcare Bio-Sciences, formerly Amersham Biosciences, describes the factors affecting the accuracy and reproducibility of gradient formation for preparative liquid chromatography (LC) and describes how to improve peak separation and process economy in chromatography applications.
  
  
• Autoclavable ultrafiltration cartridge by GE Healthcare Bio-Sciences
  In this application note, GE Healthcare Bio-Sciences describes UFP-10-B-8A ultrafiltration cartridge, which allows customized tubing to and from the cartridge to be autoclaved simultaneously, a viable alternative as a bioreactor cartridge.
  
  
• Packing Capto Q in production-scale columns by GE Healthcare Bio-Sciences
  In this application note, GE Healthcare Bio-Sciences describes methods for packing Capto Q, a strong anion exchange BioProcess medium used for packed bed chromatography, in four types of large-scale columns suitable for good manufacturing practice (GMP) manufacture: FineLINE, BPG, BioProcess LPLC and Chromaflow.
  
  
• Sanitization of Streamline direct columns by GE Healthcare Bio-Sciences
  This application note by GE Healthcare Bio-Sciences, formerly Amersham Biosciences, describes the results of two sanitization tests performed on Streamline Direct columns, which are used in expanded bed adsorption (EBA).
  
  
• Improved method of BAC DNA preparation for sequencing by GE Healthcare Bio-Sciences
  This application note by GE Healthcare Bio-Sciences, formerly Amersham Biosciences, discusses improved methods of preparing bacterial artificial chromosome (BAC) DNA for sequencing by using TempliPhi amplified BAC DNA obtained from minipreps.
  
  
• Protein sequencing of cyanogen bromide digested myoglobin electrotransferred ... by GE Healthcare Bio-Sciences
  This application note by GE Healthcare Bio-Sciences, formerly Amersham Biosciences, describes methods for analyzing fragments of cyanogen bromide digested sperm whale myoglobin with SDS-PAGE in combination with electrophoretic blotting and direct amino acid sequencing of the blotted peptides.
  
  
• Comparison of Kvick cassettes and two competitive cassettes by GE Healthcare Bio-Sciences
  In this application note, GE Healthcare Bio-Sciences, formerly Amersham Biosciences, describes a comparison study of Kvick cassettes and two competitive cassettes for the concentration and diafiltration of human serum albumin (HSA).
  
  
• Histidine-tagged proteins by GE Healthcare Bio-Sciences
  In this application note, GE Healthcare Bio-Science, formerly Amersham Biosciences describes determination of concentration range of imidazole that minimized the nonspecific binding of untagged proteins to Ni Sepharose high performance, thereby greatly improving target protein purity.
  
  
• Analysis of Clontech Atlas Human Cancer cDNA expression macroarrays using the... by GE Healthcare Bio-Sciences
  This application note by GE Healthcare Bio-Sciences, formerly Amersham Biosciences, describes the speed and inherent wide quantitative dynamic range of storage phosphor detection coupled with Storm imaging and ImageQuant analysis, an ideal solution for interpreting Clontech Atlas Human Cancer cDNA expression arrays.
  
  
• DNA analysis using PhastGel homogeneous media by GE Healthcare Bio-Sciences
  This application note by GE Healthcare Bio-Sciences, formerly Amersham Biosciences, summarizes methods that can be used with PhastSystem for the separation of oligonucleotides and double-stranded DNA and the results that can be obtained.
  
  
• Automated microfiltration using AKTAcrossflow by GE Healthcare Bio-Sciences
  In this application note, GE Healthcare Bio-Sciences, formerly Amersham Biosciences, outlines steps for optimizing the purification of βb-glucosidase from Pichia pastoris cell culture and Green Fluorescent Protein-His from an E coli cell homogenate.
  
  
• 1-μmol synthesis of DNA using ÄKTA oligopilot plus 10 by GE Healthcare Bio-Sciences
  This application note by GE Healthcare Bio-Sciences, formerly Amersham Biosciences, describes the 1-μmol synthesis of DNA using ÄKTA oligopilot plus 10, its process and results.
  
  
• Optimization of the purification of mono-PEG40 avidin by GE Healthcare Bio-Sciences
  In this application note, GE Healthcare Bio-Sciences, formerly Amersham Biosciences, describes the purification of mono-PEG40 avidin (mono-poly[ethylene] glycol 40000 avidin) using MacroCap SP cation exchange medium.
  
  
• Spot resolution and detection of proteins in 2-D electrophoresis by GE Healthcare Bio-Sciences
  This application note by GE Healthcare Bio-Sciences, formerly Amersham Biosciences, describes the effects of immobilized gradient (IPG) strip length, protein loading capacity and pH gradient on spot detection and resolution in the second dimension of electrophoresis.
  
  
• Fluorescence quantitation of PCR products using the DyNA Quant 200 by GE Healthcare Bio-Sciences
  This application note by GE Healthcare Bio-Sciences, formerly Amersham Biosciences, describes how the DyNA Quant and DQ130 Capillary Cuvette Kit can be used to determine directly the amount of polymerase chain reaction (PCR) product in an amplification mix.
  
  
• Multiple mini-format 2-D electrophoresis by GE Healthcare Bio-Sciences
  In this application note, GE Healthcare Bio-Sciences, formerly Amersham Biosciences, describes two-dimensional electrophoresis, a powerful analytical technique to resolve thousands of individual proteins in complex biological samples. The method provides convenience and reproducibility of precast gels for the first and second dimensions.
  
  
• Storm and PhosphorImager image analysis of cDNA expression macroarrays by GE Healthcare Bio-Sciences
  This application note by GE Healthcare Bio-Sciences, formerly Amersham Biosciences, describes the imaging and analysis of low-density DNA macroarrays with the Storm and PhosphorImager SI systems in order to interpret macro expression arrays.
  
  
• Two-color mutation detection by GE Healthcare Bio-Sciences
  In this application note, GE Healthcare Bio-Sciences, formerly Amersham Biosciences, describes a simple, automated method using a color complementation strategy to detect the most common mutation associated with cystic fibrosis (CF), the three-base pair deletion F508.
  
  
• Labeling sensitivity and dynamic range achievable using the HotScribe ... by GE Healthcare Bio-Sciences
  This application note by GE Healthcare Bio-Sciences, formerly Amersham Biosciences, demonstrates the sensitivity and dynamic range capabilities of the HotScribe First-Strand cDNA Labeling Kit. The kit can be used successfully with phosphate-based hybridization buffers such as Church buffer and more complex buffers such as Denhardt's.
  
  
• 384-well format ethanol precipitation method by GE Healthcare Bio-Sciences
  This application note by GE Healthcare Bio-Sciences, formerly Amersham Biosciences, describes the conditions and process of ethanol precipitation for the removal of excess dye terminators performed in a 384-well format.
  
  
• Protein mapping of mycoplasmas by GE Healthcare Bio-Sciences
  In this application note, GE Healthcare Bio-Sciences, formerly Amersham Biosciences, describes a method for analyzing cell proteins from ovine and caprine mycoplasmas, or parasites, with two-dimensional (2-D) PAGE, used as a taxonomic tool, in combination with immunoblotting.
  
  
• Chromatographic production of human plasma proteins factor VIII, albumin and IgG by GE Healthcare Bio-Sciences
  In this application note, GE Healthcare Bio-sciences describes the production, using high-pressure liquid (HPLC) chromatography, of human plasma proteins factor VIII, albumin and IgG.
  
  
• Screening for mutations of androgen receptor gene by single-strand ... by GE Healthcare Bio-Sciences
  In this application note, GE Healthcare Biosciences discusses the androgen receptor, an intracellular transcription factor that regulates sexual development, spermatogenesis in the male and single-strand conformation polymorphism (SSCP), a technique useful in screening for mutations in the androgen receptor.