High Performance Capillary Electrophoresis of Carbohydrates
by Beckman Coulter Inc.
This white paper by Beckman Coulter Inc. describes the basic aspects of the electrolyte systems used in high performance capillary electrophoresis (HPCE) of carbohydrates, to discuss the advantages and disadvantages of the approaches and concepts that are most useful in the separation and detection of carbohydrates by HPCE, and to review important applications for various types of carbohydrates. It also discusses HPCE in its various modes of separation and detection where it is suitable for the analysis of a wide variety of carbohydrate species including mono-, oligo-, and polysaccharides, glycopeptides, glycoproteins, and glycolipids and was facilitated in part by the progress made in the capillary column technology and by the introduction of novel electrolyte systems. Finally it establishes the advantages of HPCE over other separation techniques such as HPLC and traditional polyacrylamide gel electrophoresis reside in its higher separation efficiencies, shorter analysis time, small sample requirements and, more importantly, lower consumption of expensive reagents and solvents.
| Published: | 2006 |
| Format: | |
| Length: | 109 pages |
| Type: | White paper |
| Language: | English |
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