Performance characteristics of the Yeast ProtoArray PPI Proteome microarray
by Invitrogen Corp.
This white paper by Invitrogen Life Technologies describes a new paradigm for studying protein interactions on a proteome scale, the Yeast ProtoArray PPI Proteome microarray. By using this microarray, proteins of interest can be screened for interactions with the majority of the yeast proteome in as little as four hours. This paper describes both how this unique discovery platform is manufactured and rigorously quality controlled to ensure reproducible interaction screening results and an integrated system for the controlled and reproducible labeling of proteins with biotin. In further studies, it shows that using Alexa Fluor fluorescent labels to detect protein interactions on the Yeast ProtoArray PPI Proteome microarray is superior to detection using a CyDye label. Finally, results of probing with human proteins are presented that clearly demonstrate the broad utility of the ProtoArray plat form.
| Published: | 2004 |
| Format: | |
| Length: | 12 pages |
| Type: | White paper |
| Language: | English |
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