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Current challenges of real-time polymerase chain reaction quantitation

by Genzyme Corp.

This white paper by Genzyme Genetics explains current challenges of real-time polymerase chain reaction (PCR) quantitation, its applications and challenges. Real-time PCR quantitation is a powerful and multi-faceted technology platform with many applications in both research and clinical laboratory settings. Although there are many advantages to using this method over other methods, there are a number of limitations and challenges that the user must carefully evaluate as part of a comprehensive validation of any experimental and clinical protocol. These include an understanding of the chemistry, instrumentation, controls, quality control, clinical application, sample requirements, data analysis and economics.

Published: 2006
Format: PDF
Length: 8 pages
Type: White paper
Language: English
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