Optimizing reversed-phase HPLC separations
by MAC-MOD Analytical Inc.
This white paper by MAC-MOD Analytical Inc. provides guidelines for optimizing chromatographic conditions so that reversed-phase high-pressure liquid chromatography (HPLC) separations can be accomplished efficiently and successfully. A common cause of poor column efficiency in reversed-phase HPLC is interference from secondary retention that occurs when an ion-exchange interaction takes place between positively charged analytes and acidic silanols on the surface of silica stationary phase support particles. This white paper also describes gradient elution, reduces separation time for mixtures containing compounds with a wide range of K values or for the separation of mixtures containing large molecules.
| Published: | 2006 |
| Format: | |
| Length: | 6 pages |
| Type: | White paper |
| Language: | English |
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