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Histidine-tagged proteins

by GE Healthcare Bio-Sciences

In this application note, GE Healthcare Bio-Science, formerly Amersham Biosciences describes determination of concentration range of imidazole that minimized the nonspecificfic binding of untagged proteins to Ni Sepharose high performance, thereby greatly improving target protein purity.

Published: 2006
Format: PDF
Length: 4 pages
Type: Application Note
Language: English
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