A setup system for compensation
by BD Biosciences
This white paper by BD Biosciences describes compensation in multicolor flow cytometry, which is most accurately determined by measuring spectral overlap values and their subsequent conversion to compensation values by matrix algebra. It also explains controls in spectral overlap determination using fluorophores. BD Biosciences describes software that reads the fluorescence of each single-color control in sequential acquisitions and its own BD FACSDiva software, which provides automatic access to stored spectral overlap values. BD FACSDiva's instrument setup automatically determines the spectral overlap of each of the captured conjugates.
| Published: | 2006 |
| Format: | |
| Length: | 8 pages |
| Type: | White paper |
| Language: | English |
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