TaqMan gene expression assays for validating hits from fluorescent microarrays
by Applied Biosystems
Gene expression microarrays are used by nearly every major target discovery laboratory to screen for sets of genes involved in various biological processes, whether they are working with human samples or with model organisms such as mouse, rat, and drosophila. This white paper by Applied Biosystems describes TaqMan gene expression assays which are designed differently than microarrays and have no positional bias within a transcript. It also explains validation of microarray results, microarray variability, data analysis, general laboratory processes and microarray technology limitations. Finally describes historical microarray validation workflow.
| Published: | 2006 |
| Format: | |
| Length: | 6 pages |
| Type: | White paper |
| Language: | English |
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