Evaluation of precision with ultra-fast LC conditions
by Shimadzu Scientific Instruments
There is a growing trend toward the use of smaller (< 3 µm) particle size columns to reduce analysis time and increase sample throughput. Recently, a novel 2.2 µm particle size column was introduced that allows efficiencies similar to sub-2µm columns, but at greatly reduced system back-pressures. The use of this column with a conventional LC system that features high-resolution pumps (3nL/step) and a high-speed autosampler now allows higher throughput to be obtained with back-pressures similar to traditional HPLC conditions. Retention time and peak area reproducibility results for an ultra-fast LC separation will be presented.
| Published: | 2007 |
| Format: | |
| Length: | 11 pages |
| Type: | White paper |
| Language: | English |
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