Bioprocess Development

Rediscovered: The Achilles Heel of Cancer
White paper
The “Achilles heel” of cancer was discovered years ago, but abandoned due to what was believed to be a faulty experimental protocol with no practical application to treating humans, but do these tests need to be a second look?

Quality control of antibodies using the 2100 bioanalyzer and Protein 200 plus...
By Agilent Technologies
Application note
In this application note, Agilent Technologies describes the use of systems such as the 2100 Bioanalyzer and Protein 200 Plus assay to evaluate a stress stability test of two different antibodies.

Agilent 2100 bioanalyzer system—a compliant tool
By Agilent Technologies
Application note
In this application note, Agilent Technologies describes its 2100 Bioanalyzer tool and explains how it can be used for quality control and analysis of antibodies, protein pharmaceuticals and other biomolecules.

mAb Sector Growth Will Continue to Far Outstrip that of Small Molecules
White paper
Independent market analyst Datamonitor forecasts continued expansion with key individual mAb product franchises expected to record peak sales growth through horizontal indication broadening and the launch of new products in the next few years.

Debunking the Cancer Drug Conspiracy Myth
White paper
In this white paper, Craig Dees, Ph.D., CEO of Provectus, examines the conspiracies and myths surrounding cancer drugs.

Reoviruses as Cancer Therapeutics
White paper
This white paper by Matt Coffey, Ph.D., chief scientific officer, Oncolytics Biotech, discusses a new virus product, Reolysin, that aims to attack cancer cell types with only mild side effects in comparison to traditional cytotoxic cancer therapies which cause serious health risks.

A PSE approach for yield enhancement in bioprocessing
By Technion Israel Institute of Technology
White paper
This white paper, from Technion IIT, shows that a combination of improved process control in the downstream processing sections as well as a modified substrate feeding profile in the fermentor, can together achieve a 40 percent reduction in batch time while significantly increasing throughput yield for pharmaceutical manufacturing companies.

Rediscovered: The Achilles Heel of Cancer
White paper
The “Achilles heel” of cancer was discovered years ago, but abandoned due to what was believed to be a faulty experimental protocol with no practical application to treating humans, but do these tests need to be a second look?

Quality control of antibodies using the 2100 bioanalyzer and Protein 200 plus...
By Agilent Technologies
Application note
In this application note, Agilent Technologies describes the use of systems such as the 2100 Bioanalyzer and Protein 200 Plus assay to evaluate a stress stability test of two different antibodies.

Handling Bead and Cell Suspensions Using the CyBi-NanoJet
By CyBio AG
Application note
In this application-note some examples of bead and cell handling using the CyBi-NanoJet are presented.

Affordable, Efficient CD4 T Cell counting using the Guava EasyCD4 assay system
By Guava Technologies Inc.
Application note
In this application note, Guava Technologies, Inc. explains a novel technology for cell analysis that is generically referred to as microcapillary cytometry, which permits analyses to be performed on very small numbers of cells in typically only a few microliters, using far less reagent to label cells than conventional cell analysis systems.

Total RNA purification from cultured cells using the ABI PRISM 6700
By Applied Biosystems
Application note
In this application note, Applied Biosystems describes its newly developed techniques using the ABI PRISM 6700 Automated Nucleic Acid Workstation that produce high purity total RNA from biological sources such as cultured cells which eliminates the need for post-isolation DNase treatment or other manual handling, and produce a highly pure product, free of PCR inhibitors, that is less prone to...

In vitro screening for inflammatory activities of potential drug compounds
By MD Biosciences Inc.
White paper
This white paper by MD Biosciences provides information on cytokine assays for investigating the inhibition of inflammatory cytokine production by test compounds.

Bioassay Development Services
By Cardinal Health
Product collateral
<P>Cardinal Health's approach to bioassay optimization evaluates the existing assay under non-cGMP conditions at a selected end point, confirms the negative control (product), prepares and validates master or working cell banks, optimizes cell numbers and preassay conditions, defines parallelism regions, and runs under cGMP conditions. </P>

Development and validation of in-vitro assay for the release of estrogen from...
By AAI Pharma Inc.
White paper
This white paper by AAI Development Services explains the development and validation of an in-vitro release method for determination of the release of Estrasorb from a lotion.

Current challenges of real-time polymerase chain reaction quantitation
By Genzyme Corp.
White paper
This white paper by Genzyme Genetics explains current challenges of real-time polymerase chain reaction (PCR) quantitation, its applications and challenges.

A PSE approach for yield enhancement in bioprocessing
By Technion Israel Institute of Technology
White paper
This white paper, from Technion IIT, shows that a combination of improved process control in the downstream processing sections as well as a modified substrate feeding profile in the fermentor, can together achieve a 40 percent reduction in batch time while significantly increasing throughput yield for pharmaceutical manufacturing companies.

Kerasep Diamond high flux rate ceramic monoliths
By Novasep Inc.
Product collateral
Kerasep Diamond membranes by Novasep are available in a wide range of cut-offs for the separation of organic molecules, water soluble polymers, emulsions and specific inorganic products.

High Performance Capillary Electrophoresis of Carbohydrates
By Beckman Coulter Inc.
White paper
This white paper by Beckman Coulter Inc. describes the basic aspects of the electrolyte systems used in high performance capillary electrophoresis (HPCE) of carbohydrates, to discuss the advantages and disadvantages of the approaches and concepts that are most useful in the separation and detection of carbohydrates by HPCE, and to review important applications for various types of carbohydrates.

Comparison guide to C18 reversed phase HPLC columns
By MAC-MOD Analytical Inc.
White paper
This white paper by MAC-MOD Analytical Inc. is a comparison guide to C18 reversed phase high-pressure liquid chromatography (HPLC) columns.

RediSep C-18 reversed phase column: Purification of carboxylic acids
By Teledyne Isco Inc.
Application note
In this application note, Teledyne Isco, Inc. describes the purification of a mixture of carboxylic acids using Teledyne Isco’s RediSep C-18 reversed phase column as the stationary phase and it illustrates that the use of C-18 reversed phase RediSep column as a highly practical and efficient tool for the purification of carboxylic acids .

Separation of proteins and peptides by capillary electrophoresis.
By Beckman Coulter Inc.
White paper
This white paper by Beckman Coulter Inc. describes where and why CE can be used as a solution to a critical analytical need and specifically explains the role of capillary electrophoresis in product identity, quantity, purity, heterogeneity, stability, and process consistency is addressed.

Strategies for HPLC method development and validation for pharmaceutical ...
By AAI Pharma Inc.
White paper
This white paper by AAI Development Services points out challenges with combination products and offers points in developing and validating analytical methods including HPLC.

Peptide separations using reversed phase RediSep columns
By Teledyne Isco Inc.
Application note
In this application note, Isco Inc. describes how peptides are separated using reversed phased RediSep columns and outlines how peptide purification methods can be established on smaller columns, then scaled up to suit the desired sample load.

Genetic researchers score a bargain: Free delivery of DNA
By Microfluidics Corp.
Case study
In this case study, Microfluidics Corp. 's provides the University of Pittsburgh with a Microfluidizer processor that enables liposomes to carry DNA piggyback-style into a cell, a technique that speeds the regulatory approval process.

Automated SFC method development: optimization of ibuprofen separation using ...
By Thar Technologies
White paper
This white paper by Thar Technologies Inc. describes the optimization of ibuprofen separation using a superpure discovery analytical supercritical fluid chromatography (SFC) instrument.

A PSE approach for yield enhancement in bioprocessing
By Technion Israel Institute of Technology
White paper
This white paper, from Technion IIT, shows that a combination of improved process control in the downstream processing sections as well as a modified substrate feeding profile in the fermentor, can together achieve a 40 percent reduction in batch time while significantly increasing throughput yield for pharmaceutical manufacturing companies.

Rediscovered: The Achilles Heel of Cancer
White paper
The “Achilles heel” of cancer was discovered years ago, but abandoned due to what was believed to be a faulty experimental protocol with no practical application to treating humans, but do these tests need to be a second look?

Kerasep Diamond high flux rate ceramic monoliths
By Novasep Inc.
Product collateral
Kerasep Diamond membranes by Novasep are available in a wide range of cut-offs for the separation of organic molecules, water soluble polymers, emulsions and specific inorganic products.

Handling Bead and Cell Suspensions Using the CyBi-NanoJet
By CyBio AG
Application note
In this application-note some examples of bead and cell handling using the CyBi-NanoJet are presented.

Affordable, Efficient CD4 T Cell counting using the Guava EasyCD4 assay system
By Guava Technologies Inc.
Application note
In this application note, Guava Technologies, Inc. explains a novel technology for cell analysis that is generically referred to as microcapillary cytometry, which permits analyses to be performed on very small numbers of cells in typically only a few microliters, using far less reagent to label cells than conventional cell analysis systems.

Total RNA purification from cultured cells using the ABI PRISM 6700
By Applied Biosystems
Application note
In this application note, Applied Biosystems describes its newly developed techniques using the ABI PRISM 6700 Automated Nucleic Acid Workstation that produce high purity total RNA from biological sources such as cultured cells which eliminates the need for post-isolation DNase treatment or other manual handling, and produce a highly pure product, free of PCR inhibitors, that is less prone to...

Benefits of monitoring CO2 and O2 in pharmaceutical fermentation processes
White paper
As aerobic fermentation progresses, oxygen is consumed and carbon dioxide is relased by the cultures present, but the rates can vary enormously. In this white paper by Servomex Inc., the advantages of careful monitoring of both are discussed.

Solvent-resistant stirred cells for ultrafiltration and filtration applications
By Millipore Corp.
Application note
This application note by Millipore Corp. describes the principles behind its solvent-resistant stirred cell technique and its applications, including the removal or concentration of macrosolutes and the removal or exchange of buffers, solvents and microsolutes.

Carbosep Membranes
By Novasep Inc.
Product collateral
Carbosep membranes by Novasep are available in a wide range of cut offs and trilobe membranes offer high flux rates with reduced numbers of membranes providing a more compact design.

Hynetics one touch bioprocessing systems
By Alfa Laval Biokinetics
White paper
This white paper by Alfa Laval Biokinetics Inc. describes the Hynetics one touch bioprocessing system, a formulation system for manufacturing media and other sterile process liquids.