Sourcing and Services

CHO cell culture application using a BioFlo 110 benchtop bioreactor
By New Brunswick Scientific Co.
Application note
In this application note, New Brunswick Scientific Co. discusses simple protocols for culturing cells in a BioFlo 110 benchtop fermentor/bioreactor system and concentrates on achieving high-density CHO cell cultures using its Advanced Cell Culture Kit.

Time-of-flight solutions in pharmaceutical development
By Agilent Technologies
Application note
In this application note, Agilent Technologies explores the collection of examples and applications based on the Agilent liquid chromatography/mass-selective detector (LC/MSD) time-of-flight mass spectrometer and focuses on the power of accurate mass in both qualitative and quantitative applications.

Validated HPLC columns
By MAC-MOD Analytical Inc.
Application note
In this application note, MAC-MOD Analytical Inc. describes a method of validating high-performance liquid chromatography (HPLC) columns that assures constant column performance over the lifetime of an analytical method.

Comparison of Kvick cassettes and two competitive cassettes
By GE Healthcare Bio-Sciences
Application note
In this application note, GE Healthcare Bio-Sciences, formerly Amersham Biosciences, describes a comparison study of Kvick cassettes and two competitive cassettes for the concentration and diafiltration of human serum albumin (HSA).

Histidine-tagged proteins
By GE Healthcare Bio-Sciences
Application note
In this application note, GE Healthcare Bio-Science, formerly Amersham Biosciences describes determination of concentration range of imidazole that minimized the nonspecific binding of untagged proteins to Ni Sepharose high performance, thereby greatly improving target protein purity.

Analysis of Clontech Atlas Human Cancer cDNA expression macroarrays using the...
By GE Healthcare Bio-Sciences
Application note
This application note by GE Healthcare Bio-Sciences, formerly Amersham Biosciences, describes the speed and inherent wide quantitative dynamic range of storage phosphor detection coupled with Storm imaging and ImageQuant analysis, an ideal solution for interpreting Clontech Atlas Human Cancer cDNA expression arrays.

Multiple mini-format 2-D electrophoresis
By GE Healthcare Bio-Sciences
Application note
In this application note, GE Healthcare Bio-Sciences, formerly Amersham Biosciences, describes two-dimensional electrophoresis, a powerful analytical technique to resolve thousands of individual proteins in complex biological samples. The method provides convenience and reproducibility of precast gels for the first and second dimensions.

Two-color mutation detection
By GE Healthcare Bio-Sciences
Application note
In this application note, GE Healthcare Bio-Sciences, formerly Amersham Biosciences, describes a simple, automated method using a color complementation strategy to detect the most common mutation associated with cystic fibrosis (CF), the three-base pair deletion F508.

384-well format ethanol precipitation method
By GE Healthcare Bio-Sciences
Application note
This application note by GE Healthcare Bio-Sciences, formerly Amersham Biosciences, describes the conditions and process of ethanol precipitation for the removal of excess dye terminators performed in a 384-well format.

Protein mapping of mycoplasmas
By GE Healthcare Bio-Sciences
Application note
In this application note, GE Healthcare Bio-Sciences, formerly Amersham Biosciences, describes a method for analyzing cell proteins from ovine and caprine mycoplasmas, or parasites, with two-dimensional (2-D) PAGE, used as a taxonomic tool, in combination with immunoblotting.